Table of Contents Author Guidelines Submit a Manuscript
International Journal of Cell Biology
Volume 2015, Article ID 369874, 9 pages
http://dx.doi.org/10.1155/2015/369874
Research Article

Intermittent Compressive Stress Enhanced Insulin-Like Growth Factor-1 Expression in Human Periodontal Ligament Cells

1Research Unit of Mineralized Tissue, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand
2Graduate Program in Oral Biology, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand
3Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand
4Department of Oral Medicine, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand

Received 27 February 2015; Revised 19 May 2015; Accepted 20 May 2015

Academic Editor: Rony Seger

Copyright © 2015 Jittima Pumklin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

HPDLs were seeded in 6-well plates at a density of 3×105 cells per well for applying the force and 24-well plates at density of 5×104 cells per well for being treated with CoCl2. Subsequently cells were starved with serum-free media 4 h before treatment. At 24 h, HPDLs were incubated with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide solution for 30 min. Formazan crystals were solubilized in DMSO/glycine buffer solution (0.1 M glycine/0.1 M sodium chloride pH10). The solution was further measured for an absorbance at 570 nm in a microplate reader (Elx800, Biotek, USA). The data were normalized to the control. All measurements were done in triplicate.

  1. Supplementary Material