Impact of Hybrid and Complex N-Glycans on Cell Surface Targeting of the Endogenous Chloride Cotransporter Slc12a2

Figure 7

NKCC1 localizes in cis/medial-Golgi cisternae. ((a)–(c)) Shown are representative images of COS7 cells grown under control conditions or treated for 16 h with 1 μg/mL of brefeldin A (BFA, (d)–(f)). Immunoreactive α-mannosidase II (Man2, (a) and (d)) and NKCC1 ((b) and (e)) were detected by using primary antibodies directed against these proteins and developed using secondary antibodies labeled with FITC (green) and Cy3 (red), respectively. ((c) and (f)) Shown are superimposed images to obtain an overlay representing colocalization of NKCC1 and Man2. The scale bar represents 10 μm. ((g)-(h)) Heat-maps of the squared cells in (c) and (f), respectively, computed by using NIH ImageJ.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)