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International Journal of Cell Biology
Volume 2015, Article ID 630246, 8 pages
http://dx.doi.org/10.1155/2015/630246
Research Article

Modulation Effects of Curcumin on Erythrocyte Ion-Transporter Activity

Department of Biochemistry, University of Allahabad, Allahabad 211002, India

Received 9 June 2015; Accepted 13 August 2015

Academic Editor: Richard Tucker

Copyright © 2015 Prabhakar Singh and Syed Ibrahim Rizvi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Curcumin ((1E,6E)-1,7-Bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione), the yellow biphenolic pigment isolated from turmeric (Curcuma longa), has various medicinal benefits through antioxidation, anti-inflammation, cardiovascular protection, immunomodulation, enhancing of the apoptotic process, and antiangiogenic property. We explored the effects of curcumin in vitro (10−5 M to 10−8 M) and in vivo (340 and 170 mg/kg b.w., oral) on Na+/K+ ATPase (NKA), Na+/H+ exchanger (NHE) activity, and membrane lipid hydroperoxides (ROOH) in control and experimental oxidative stress erythrocytes of Wistar rats. As a result, we found that curcumin potently modulated the membrane transporters activity with protecting membrane lipids against hydro-peroxidation in control as well as oxidatively challenged erythrocytes evidenced by stimulation of NKA, downregulation of NHE, and reduction of ROOH in the membrane. The observed results corroborate membrane transporters activity with susceptibility of erythrocyte membrane towards oxidative damage. Results explain the protective mechanism of curcumin against oxidative stress mediated impairment in ions-transporters activity and health beneficial effects.