Hyaluronan Synthase 3 Null Mice Exhibit Decreased Intestinal Inflammation and Tissue Damage in the DSS-Induced Colitis Model

Figure 7

Immunohistochemistry of DSS-treated mice. (a) Adjacent rectal sections to the H&E stained tissues presented in Figure 6 were stained for hyaluronan (green) with the biotinylated hyaluronan binding protein (HABP) and Alexa Fluor streptavidin-488. Nuclei were stained with DAPI (blue). Images are 10x magnification captured with a Leica digital fluorescent microscope and ImageProPlus software. (b) Semiquantitative densitometric analysis of HA staining in longitudinal folds in the images presented in panel (a). The untreated wild-type rectal section illustrates the regions measured (yellow). Lamina propria HA staining (LP) is the region above the muscularis mucosae while submucosa HA staining (SM) is the area below the muscularis mucosae and above the muscularis externa.

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