Research Article

High Sensitivity Method to Estimate Distribution of Hyaluronan Molecular Sizes in Small Biological Samples Using Gas-Phase Electrophoretic Mobility Molecular Analysis

Figure 1

GEMMA data profile. Molecular size distribution of HA extracted from four different tissues and species, rat kidney (orange), chicken comb (green), human heart (blue), and moose heart (red), one of each. The electroforetic molecule diameter analyzed in the GEMMA was converted to molecular weight by analyzing HA standards from Hyalose L.L.C. ranging from 30 kDa to 2400 kDa samples [21]. HA from moose heart and chicken comb both showed uniform HMW HA peaks around ca 5000 kDa. HA from rat kidney showed the HMW peak and an additional broad peak with a center around 30 kDa. Human heart also showed presence of LMW HA in the same range as in rat kidney but the HMW HA content was of apparent greater size than the other three examples shown.