Carbohydrate Moieties and Cytoenzymatic Characterization of Hemocytes in Whiteleg Shrimp Litopenaeus vannamei

Table 1

Lectins used to investigate cell surface oligosaccharides domains in hemocytes and their competitive sugars used as controls for lectin-binding.

Lectin	Latin name	Source	Resuspension solution	Specificity sugar	Inhibition
Con-A	Canavalia ensiformis; (Sigma, C7642)	Jack bean	NaCl 0.9% pH 6.5 with 5 mM CaCl2	a-D-Mannose, a-D-glucose	Glucose
WGA	Triticum vulgaris; (Sigma, L4895)	Wheat germ	PBS pH 7.4	(D-GlcNAc), NeuNAc	N-Acetylglucosamine
PNA	Arachis hypogaea; (Sigma, L7381)	Peanut	PBS pH 7.4	-Galactose(1-3) galNAc	Galactosamine
UEA-I	Ulex europaeus; (Sigma, L9006)	Gorse, furze	NaCl 0.9%	a-L-Fucose	Fucose
LEA	Lycopersicon esculentum; (Sigma, L0401)	Tomato		(glcNAc)3	N-Acetylglucosamine

GalNAc = N-acetyl-galactosamine, GlcNAc = N-acetyl-glucosamine, and NeuNAc = N-acetylneuraminic acid.
Competing sugar used in the study at 0.2 M.
10 mM HEPES, 0.15 M NaCl, 0.1 mM Ca²⁺, 0.08% sodium azide, and 5 mg mL⁻¹   β-cyclodextrin.