Exploring Seipin: From Biochemistry to Bioinformatics Predictions

Figure 8

Biological integration. A network with genes differently regulated in seipin loss-of-function situations was created using STRING [41]. The gene list submitted is in accordance with Supplementary Tables 1 and 2. Genes painted with green are upregulated in adipogenic cells with seipin loss-of-function. Genes painted with blue are downregulated in adipogenic cells with seipin loss-of-function. Genes painted in gray are upregulated in nonadipogenic cells with seipin loss-of-function and genes painted in pink are downregulated in nonadipogenic cells with seipin loss-of-function. Genes that were observed either downregulated or upregulated in the same cell situations but in different papers are painted in yellow (mixed expression). Proteins already described as seipin physical binders are surrounded by a red circumference. The parameters chosen were “Experiments,” “Databases,” “Coexpression,” “Neighborhood,” “Gene Fusion,” “Cooccurrence,” and “Minimum required interaction score = 0.1.” Cytoscape [42] classifies the genes based on their connectivity. As the circle comes to the center, the node tends to be more connected with the network.