Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation

Figure 1

hDPSC surface markers and morphology of the cultures. The hDPSC were isolated from dental pulp of permanent teeth from three healthy donors. (a) The analysis by flow cytometry showed a homogeneous cell population positive for mesenchymal markers CD90, CD105, and CD73 and negative for early hematopoietic markers CD34, CD45, CD14, and CD20. Cells from fourth passage were recorded of each sample in triplicate. Data correspond to a representative experiment. (b) The hDPSC showed spindle-shape or fibroblast-like morphology with adherence to the surface and colony formation ability growing in a swirling-like pattern. During the differentiation process (OLC) and in the EXP-21, the cells maintained a typical fibroblastic-like shape with long cytoplasmic processes and were orderly arranged with a tendency to align themselves in parallel lines. The EXP-21 cells after cryopreservation, storage, and thawing showed similar morphology than their regular noncryopreserved cells. Scale bar: 200 μm.

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