Research Article
Odontoblast-Like Cells Differentiated from Dental Pulp Stem Cells Retain Their Phenotype after Subcultivation
Table 1
Primers used in this study.
| | Gene bank | Name | Forward | | Reverse |
| | NM_001015051.3 | RUNX2 | 5′-CATCTAATGACACCACCAGGC-3′ | | 5′-GCCTACAAAGGTGGGTTTGA-3′ | | NM_001040058.1 | OPN | 5′-TGAAACGAGTCAGCTGGATGACCA-3′ | | 5′-TGGCTGTGAAATTCATGGCTGTGG-3′ | | NM_001173467.1 | OSX | 5′-TGGGAAAAGGGAGGGTAATC-3′ | | 5′-CGGGACTCAACAACTCTGG-3′ | | NM_000478.4 | ALP | 5′-TCAGAAGCTCAACACCAACG-3′ | | 5′-GTCAGGGACCTGGGCATT-3′ | | NM_000088.3 | COLI | 5′-TGACCTCAAGATGTGCCACT-3′ | | 5′-ACCAGACATGCCTCTTGTCC-3′ | | NM_014208.3 | DSPP | 5′-GGCAGTGCATCAAAAGGAGC-3′ | | 5′-TGCTGTCACTGTCACTGCTG-3′ | | NM_004407.3 | DMP-1 | 5-TGGAGTTGCTGTTTTCTGTAGAG-3 | | 5′-ATTGCCGACAGGATGCAGA-3′ | | NM_001101.3 | β-ACT | 5′-ATTGCCGACAGGATGCAGA-3′ | | 5′-GAGTACTTGCGCTCAGGAGGA-3′ |
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RUNX2: runt-related transcription factor 2; OPN: osteopontin; OSX: osterix; ALP: alkaline phosphatase; COL-I: collagen type I; DSPP: dentin sialophosphoprotein; DMP-1: dentin matrix protein-1; β-ACT: β-actin.
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