Research Article

Engineering of L-Plastin Peptide-Loaded Biodegradable Nanoparticles for Sustained Delivery and Suppression of Osteoclast Function In Vitro

Figure 5

MTT assay and TRAP-staining of osteoclasts after treatment with TAT-fused sNT-LPL peptides (P1 and P3) and nanoparticles (NP1 and NP3). Osteoclasts were treated with indicated peptides or nanoparticles for 4h in the presence of TNF-α and bone particles. Next, cells were subjected to the calorimetric MTT assay as described in the Methods to determine the proliferation effects (a). TRAP staining was performed to determine the cell morphology or phenotype of osteoclasts (b). Osteoclasts untreated with peptides but treated with TNF-α and bone particles were used as controls (-). MTT assay confirmed that proliferation activity is not affected. Morphological characterization by TRAP-staining and attachment of osteoclasts to culture dishes suggest that cells are active and viable. Magnification-40X. These results represent one of the two experiments performed with similar results.

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