Engineering of L-Plastin Peptide-Loaded Biodegradable Nanoparticles for Sustained Delivery and Suppression of Osteoclast Function In Vitro

Figure 8

Schematic representation of the effects of small MW L-plastin (LPL) peptides on osteoclast bone resorption. Biodegradable and biocompatible PLGA-PEG nanocarriers were used to deliver and release the small molecular weight L-plastin peptides (NP1 or NP3) of interest in a controlled and sustained fashion. Osteoclasts were incubated with these nanoparticles of interest in the presence of TNF-α. The released peptide P1 attenuated TNF-α induced phosphorylation of cellular LPL. Therefore, the formation of NSZs and sealing rings as well as resorption activity are significantly reduced (red arrows) in osteoclasts in vitro. No inhibition was found with the control peptide (P3). Sealing ring (SR) is indicated with an arrow in C.