International Journal of Carbohydrate Chemistry

Research Article

Effect of Molecular Sizes of Chondroitin Sulfate on Interaction with L-Selectin

Table 3

Kinetic parameters for the interaction of fractionated chondroitin sulfate with immobilized L-selectin.


	Fraction	(M⁻¹ s⁻¹)	(s⁻¹)	(M⁻¹)	(μM)

Whale cartilage	CS (untreated)	6.16 × 10²	7.52 × 10⁻³	8.18 × 10⁴	12.2
	B (21 kDa)	7.58 × 10³	2.27 × 10⁻²	3.34 × 10⁵	3.0
	C (15 kDa)	2.31 × 10³	2.05 × 10⁻²	1.13 × 10⁵	8.8
	E (9 kDa)	6.05 × 10²	3.42 × 10⁻²	1.77 × 10⁴	56.4

Bovine tracheal cartilage	CS (untreated)	3.32 × 10²	1.30 × 10⁻²	2.56 × 10⁴	39.1
	C (21 kDa)	7.97 × 10²	3.48 × 10⁻³	2.23 × 10⁵	4.5
	E (15 kDa)	1.22 × 10²	2.30 × 10⁻³	5.29 × 10⁴	18.9
	G (9 kDa)	4.52 × 10²	2.37 × 10⁻²	2.00 × 10⁴	49.9

The association rate constant (), dissociation () rate constant, equilibrium association constant () and equilibrium dissociation constant () for the interaction of CS with immobilized L-selectin were determined by BIAcore. Standard errors of kinetic parameters obtained were within 15% by tripricate experiments.