Table of Contents
International Journal of Carbohydrate Chemistry
Volume 2014 (2014), Article ID 287281, 12 pages
http://dx.doi.org/10.1155/2014/287281
Research Article

Twenty Traditional Algerian Plants Used in Diabetes Therapy as Strong Inhibitors of α-Amylase Activity

Laboratoire des Sciences Fondamentale, Université Amar Telidji, Laghouat, Algeria

Received 1 May 2014; Revised 7 July 2014; Accepted 8 July 2014; Published 5 August 2014

Academic Editor: Jianjun Li

Copyright © 2014 Ihcen Khacheba et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the present work, we have studied the inhibitory effects of aqueous and alcoholic extracts of six Algerian medicinal plants known by their therapeutic virtues against diabetes. The total phenolic compounds content, assayed using Folin-Ciocalteu’s reagent, of the samples ranged from 0.183 mg/g to 43.088 mg/g and from 1.197 mg/g to 7.445 mg/g, expressed as gallic acid equivalent (GAE), for the, respectively, whereas the total flavonoids concentrations, detected using 2% of the aluminium chloride, ranged from 0.41 mg/g to 11.613 mg/g and from 0.0097 mg/g to 1.591 mg/g, expressed as rutin equivalents (RE), for the aqueous and methanolic extracts, respectively. The major plants were found to inhibit enzymatic activities of Aspergillus oryzae-amylase in a concentration dependent manner. The values of the inhibition constants ( ) have been determined according to the Dixon and Lineweaver-Burk methods. The results showed that the values were less than 55 ppm for the all extracts. A strong inhibition was found in the phenolic extract of Salvia officinalis with a of 8 ppm.