Antioxidant Activity and Volatile Composition of Red Araçá Pulp Under Different Drying Conditions

Bombardelli, Michele C. M.; Johann, Gracielle; Machado, Christiane S.; Torres, Yohandra R.; Palú, Fernando; Oliveira, Kamila C.; Silva, Edson A.

doi:https://doi.org/10.1155/2019/9836929

International Journal of Chemical Engineering

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Abstract Introduction Materials and Methods Results and Discussion Conclusions Data Availability Conflicts of Interest Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2019 | Article ID 9836929 | https://doi.org/10.1155/2019/9836929

Antioxidant Activity and Volatile Composition of Red Araçá Pulp Under Different Drying Conditions

Michele C. M. Bombardelli,¹Gracielle Johann,²Christiane S. Machado,³Yohandra R. Torres,³Fernando Palú,⁴Kamila C. Oliveira,⁴and Edson A. Silva⁴

Academic Editor: Gianluca Di Profio

Received14 Nov 2018

Accepted13 Jan 2019

Published03 Feb 2019

Abstract

Araçá fruit extracts were dried at different air conditions, and an investigation of the impact of drying on the volatile composition and antioxidant activity of araçá extracts was conducted. The effective moisture diffusivity varied between 8.542 × 10⁻⁸ and 13.34 × 10⁻⁸ m²/min. Fruit extracts dried at 50°C and 2.0 m/s had the highest total antioxidant activity (1916.10 mg_{ascorbic acid}/100 g_araçá). The highest phenolic content (556.28 mg_GAE/100 g_araçá) was obtained when fruits were dried at 40°C and 1.5 m/s, but the resulting extract contained high amounts of 5-hydroxymethylfurfural (HMF), a contaminant formed in sugar-rich foods as a result of heating. Araçá extracts had similar qualitative profiles of volatile compounds by GC-MS, with caryophyllene being the most abundant terpene, followed by 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, selina-3,7(11)-diene, γ-terpinene, γ-cadinene, and α-salinene. HMF corresponded to the major peak in all chromatograms, proving that thermal drying affected the quality of the extracts.

1. Introduction

Psidium cattleianum Sabine belongs to the Myrtaceae family and is commonly referred to as strawberry guava or araçá. This plant is distinguished from the common guava, P. guajava, by its more attractive foliage and fruit. Araçá berries, which can be yellow or red, have a nice balance between soluble solids and acidity, and araçá ripens in Brazil in late summer, between February and May [1]. The ripe fruit can be eaten raw or used to prepare juices, wine, and liqueurs. Other studies from the literature have indicated that antioxidant activity, phenolic content, and different carotenes may vary according to the fruit genotypes [2, 3]. Furthermore, knowing the antioxidant activity and bioactive compounds content of different fruit genotypes may aid the valorization of natural products that increase the protection against cellular oxidation or have antimicrobial or anticarcinogenic activities [4]. In the literature, a small number of research papers have analyzed the chemical composition and antioxidant activity of araçá. In these studies, different solvents were employed to obtain the pulp extract, such as diethyl ether [5], acetone/water [6], deionized water and acetone [7], hexane, ethyl acetate, and methanol [8], and ethanol [5]. Then, the antioxidant capacity was measured by using two major methods, the DPPH (2,2-diphenyl-1-picryl-hydrazylhydrate) radical photometric method, conducted in the presence of methanol [7, 9], and the Trolox equivalent antioxidant capacity method [6, 8]. The results for the red araçá pulp while varying the extraction method and the method used to determine the antioxidant capacity were 29.29 to 37.69 mg/100 g and 48.05 μg/mL [7, 9] and 1 to 47 and 11.3 μmol Trolox g⁻¹ fresh weight [6, 8]. Moreover, in the literature, the volatile composition of red araçá pulp was analyzed by GC-MS using hydrogen gas at 280°C, resulting mostly in ethanol, α-pinene, (Z)-3-hexenol, (E)-β-caryophyllene, and hexadecanoic acid [5]. In spite of that, in the literature, there is no mention of the influence of drying conditions on these properties. Thermal treatments can affect the quality of the raw material due to the reduction of volatile compounds or the formation of new compounds by oxidation or esterification reactions [10]. Therefore, the effect of drying on the chemical composition of dried fruit extracts is of vital technological and nutritional importance [11]. In view of the above, the present work aimed at mathematically modeling the thin-layer drying of araçá and at evaluating possible alterations of antioxidant activity and volatile composition of red araçá extracts submitted to different convective drying conditions.

2. Materials and Methods

2.1. Sample Preparation

Red araçá (Psidium cattleianum Sabine) fruits were collected in the region of Guarapuava (State of Paraná, Brazil). The fruits were harvested ripe (giving preference to the red ones), sanitized (sodium hypochlorite 200 ppm), and cut into slices measuring 0.7 ± 0.15 cm thickness, 1.6 ± 0.21 cm width, and 3.0 ± 0.18 cm length. The slices were stored in plastic bags (polyethylene) of approximately 1 kg, packed in a vacuum-bag, and frozen (−18°C) until the moment of drying. The moisture content (%) of the samples at the beginning and at the end of the drying tests was determined by the gravimetric method: the samples were dried at 105°C until constant weight (difference <0.001 g). The analysis was done in triplicate, and the results were expressed as mean ± standard deviation.

In order to obtain the kinetic data required for the thin-layer convective drying modeling, the samples were dried in a convective drying system. The samples’ mass throughout the drying process was weighed on a digital scale at 10 min intervals until equilibrium (i.e., difference between measurements <0.001 g). The effects of air temperature and velocity on the drying kinetics were evaluated according to a complete factorial design, performed in triplicate at the central point (Table 1).

The antioxidant activity was the selected response for each extract obtained according to the experimental design outlined in Table 1.

2.2. Thin-Layer Drying Modeling

The diffusion equation, which represents the mass balance for the araçá slices over drying, was written in Cartesian coordinates as follows:where is the araçá pulp’s moisture content on dry basis (d.b.), is the effective mass diffusivity (m²/min), and is the spatial coordinate (m).

One initial condition was required to solve the partial differential equation, Equation (1), assuming that the initial moisture distribution in the slices was uniform:where is the slices’ initial moisture (d.b.) and is the slices’ thickness (m).

The boundary conditions consist of the Neumann and Dirichlet conditions:where is the equilibrium moisture content (d.b.).

The governing equation was discretized in relation to space by finite differences, resulting in a system of ordinary differential equations (ODEs). The dsolve routine of Maple 13 software (Waterloo Maple Inc.) was used to solve the ODE system.

The effective mass diffusivity, , was estimated by the nonlinear simplex optimization method, using the NLPSolve command. The estimation was conducted so that the parameter was constant at each drying temperature, that is, independent of the dryer’s operation speed. The resulting minimized equation waswhere is the objective function, is the number of experimental data, is the experimental average moisture content of the fruit (d.b.), and is the calculated average moisture content of the fruit (d.b.).

2.3. Extraction of Bioactive Compounds

Red araçá samples dried under different conditions were subjected to ethanol extraction according to the methodology described by Ribeiro et al. [12], with some adaptations. Approximately 4.0 g of dried red araçá were weighed, and absolute ethanol was added at a 1 : 10 (w/v) mass : solvent ratio. The extraction was performed in a shaker at 120 rpm for 4 h at room temperature (25°C) and protected from light. The mixture was filtered (11 µm), and the solvent was evaporated under reduced pressure at 40°C. The concentrated material was solubilized in 4 mL of ethanol and stored in an amber bottle in a freezer (−18°C) until the determination of antioxidant activity was completed.

2.4. Antioxidant Capacity

The antioxidant activity was determined following the procedure described by Mensor et al. [13] using 1,1-diphenyl-2-picrylhydrazyl (DPPH) as reagent. Starting from the stock solution (100 µL/mL) of ethanolic extract, solutions with final concentrations ranging from 5 to 25 μL/mL were prepared. Aliquots of 2.5 mL of each extract solution were transferred to test tubes protected from light, and 1 mL of a recently prepared DPPH ethanolic solution (0.3 mM) was added into each tube. The solutions were well mixed and left to react in the dark for 30 min at room temperature. The absorbance (abs_sample) was then measured at 517 nm using a spectrophotometer (Bel Photonics, 2000UV). The blank was prepared by mixing 1 mL ethanol with 2.5 mL extract, and the negative control was prepared by mixing 1 mL of DPPH solution and 2.5 mL of ethanol. The absorbance of these solutions was also measured (abs_blank and abs_control). The spectrophotometer was zeroed with ethanol (99.5%), and ascorbic acid was used as the positive control (standard). The results were obtained in triplicate, and the antioxidatant activity (AA) was calculated from the following equation:

This activity was expressed as the half-maximum inhibitory concentration (IC₅₀), which was defined as the concentration (μg/mL) of extract required to inhibit the production of radicals by 50%.

The total antioxidant content was determined by the spectrophotometric method involving phosphomolybdenum reductions as described by Prieto et al. [14]. The method is based on the reduction of Mo⁺⁶ to Mo⁺⁵ with subsequent formation of a phosphate-Mo⁺⁵ complex, which presents maximum absorption at 695 nm. A 0.04 mL aliquot of the extracts (0.1 g/mL) was combined with 3.0 mL of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate, and 4 mM ammonium molybdate). The samples were incubated at 368.15 K for 90 min. After the samples were cooled to room temperature, they were centrifuged and the solution’s absorbance was measured at 695 nm against a blank (3.0 mL reagent solution and 0.04 mL ethanol). The results were expressed as mg_{ascorbic acid}/100 g_araçá (dry weight).

The total phenolic content (TPC) was determined by the Folin–Ciocalteau method as described by Singleton and Rossi [15]. The total phenolic assay was performed as a measure of the reducing capacity of the sample. The reaction mixture, composed of 0.1 mL of extracts (0.1 g/mL), 7.9 mL of distilled water, 0.5 mL of Folin–Ciocalteau reagent (a mixture of phosphomolybdate and phosphotungstate), and 1.5 mL of 20% (m/v) sodium carbonate, was poured into opaque flasks. The flasks were shaken and left to stand for 2 h, and the absorbance was measured at 765 nm. The TPC was calculated according to a standard curve previously prepared with gallic acid as standard. The results were expressed as milligrams of gallic acid equivalent (GAE) per 100 g of araçá (dry weight) (mg GAE/100 g of araçá).

2.5. GC-MS Analyses

All extracts were analyzed by GC-MS (Agilent 7890A and Agilent 5975C). A nonpolar capillary column HP-5MS (30 m × 0.25 mm i.d., 0.25 µm film thickness, 5% phenyl/95% dimethylpolysiloxane) was used. Helium was the carrier gas, and the injection volume was 1.0 µL. For the column, an initial temperature of 50°C was maintained for 1 min and was then increased at a rate of 2°C/min until 200°C, which was maintained for 1 min. Source and quadrupole temperatures were 230°C and 150°C, respectively. The mass spectrometer was programmed to scan from 40.0 to 1000.0 m/z. The compounds were tentatively identified based on the database of the NIST-11 library by comparing their retention indexes (RIs) to a homologous series of n-alkanes (C8–C40) and through comparison with MS data presented in the literature. The other data processing approach used was the AMDIS (Automated Mass Spectral Deconvolution and Identification System) software. Linear retention indexes were calculated according to the following equation:where is the carbon number of the standard alkane to the left of the peak under evaluation, is the carbon number of the standard alkane to the right of the peak under evaluation, is the retention time (min) of the peak under evaluation, is the retention time of the standard alkane on the left, and is the retention time of the standard alkane on the right.

2.6. Statistical Analysis

The results were statistically analyzed using the software Statistica 7.0 (Analytical Software, Tallahassee, FL, USA), and each response was evaluated independently. Values less than 0.05 () were considered statistically significant. Principal components analysis (PCA) was undertaken to compare the extracts obtained at different drying conditions and to verify the relationship among samples and their volatile compounds, total phenolic content, and antioxidant properties.

3. Results and Discussion

3.1. Thin-Layer Drying Modeling

The effective mass diffusivity was estimated simultaneously with the numerical resolution of the mathematical model. The initial moisture content of the fresh araçá used in the model as the initial condition was 79.54% ± 0.54% (d.b.).

The estimated effective mass diffusivity, , for each drying condition is listed in Table 2.

Based on the results from Table 2, an Arrhenius-type dependence was observed between the drying temperature and the effective mass diffusivity of the red araçá fruit. ranged from 8.542 × 10⁻⁸ to 13.34 × 10⁻⁸ m²/min, in accordance with the values found in the literature for other fruits, such as pineapple [16], banana [11], and fig [17]. Figure 1 shows the experimental and simulated drying kinetics for all three drying temperatures (40, 50, and 60°C).

According to Figure 1, the higher the drying temperature, the shorter the time required to reach the moisture equilibrium between the araçá fruits and the drying air. However, air velocity did not impact on the drying time as occurred with air temperature since the material does not exhibit a constant drying rate.

Even though the increase of drying air velocity provides higher mass transfer convective coefficients, the increase of air velocity from 1.5 to 2.5 m/s did not promote acceleration of the drying rate. This indicates that air velocities around 1.5 m/s were sufficient to guarantee a low resistance to mass transfer on the surface of the araçá’s slices. Accordingly, air velocities around 1.5 m/s should be used to reduce the energy demand of the process in the range of velocities studied.

The coefficients of determination indicated that more than 97% of the mean moisture variation of the araçá slices was explained by the proposed model, under the conditions studied. The final product had a moisture content of 13.58% ± 0.40% (d.b.).

3.2. Antioxidant Capacity

The extracts obtained from the dried araçá samples were evaluated for antioxidant activity by the DPPH method, total antioxidant content by the phosphomolybdenum reducing method, and total phenolic compound content. The results are expressed as mean ± standard deviation (Table 3).

Figures 2–4 show the effects of air temperature and velocity on the IC₅₀, antioxidant activity and total phenolic compounds of the extracts, respectively.

According to the IC₅₀ results (Table 3), the sample dried at 50°C and 2.0 m/s (central point of the factorial design) yielded the highest antioxidant activity (1265.21 μg_araçá/mL). The IC₅₀ results were positively affected by the drying temperature and negatively affected by the air velocity. It is worth noting that low IC₅₀ values are desired since they indicate higher antioxidant activity (Figure 2).

The best drying condition combines mild air temperatures and velocities. Denardin et al. [9] evaluated ethanolic extracts obtained from red araçá and found an IC₅₀ of 48.05 μg/mL. In our study, IC₅₀ was calculated based on the amount of dried fruit used in the extraction rather than on the quantity of extract, as was done in other studies, so higher IC₅₀ values were expected.

The total antioxidant activity (TAA) obtained for the extracts ranged from 1165.42 to 1916.10 mg_{ascorbic acid}/100 g_araçá. Both air temperature and air velocity had a significant effect on the TAA, as had the interaction between these variables () (Figure 3).

By lowering the drying temperature and increasing the air velocity, it was possible to maximize the antioxidant activity within the studied range of process conditions. These results confirm that temperature reduces the TAA of fruits due to either the destruction of phenolic compounds [18] or by structural changes. Higher air velocities may increase the mass transfer rates, causing less damage to the compounds responsible for the antioxidant activity [19].

The combination of the studied variables was also significant, and the highest TAA (1916.10 mg_{ascorbic acid}/100 g_araçá) was obtained at the central point condition, 50°C and 2.0 m/s. This TAA is much higher than the value found by Medina et al. [7], 24 mg/100 g for red araçá and 7.2 mg/100 g for yellow araçá.

The ethanolic extracts obtained from the dried samples yielded TPCs from 347.23 to 556.28 mg_GAE/100 g_araçá, which classify them as extracts of medium and high TPC [20]. The highest amount of phenolic compounds was found for the extracts dried at 40°C and 1.5 m/s (556.28 mg_GAE/100 g_araçá) and at 50°C and 2.0 m/s (542.68 mg_GAE/100 g_araçá). The air velocity and drying temperature alone had no significant effect on the TPC (), but the interaction between these variables had a positive and significant effect (Figure 4).

Except for run 4 (60°C and 2.5 m/s), the increase in drying temperature and air velocity was followed by a decrease in TPC. This behavior is related to the sensitivity of these metabolites to temperature and to the start of degradation in the presence of oxygen.

The results of TPC found in this work were similar to those from Luximon-Ramma et al. [6], 563 mg_GAE/100 g. Although the fruits used in both studies were from the same species, the difference in phenolic composition is not surprising as the extraction protocol and the identification methods were different. The TPCs determined in this work using ethanol as extraction solvent for the araçá fruits were close to the values found by Medina et al. [7], 581.02 to 768.21 mg_GAE/100 g_{fresh fruit}.

Neri-Numa et al. [21] evaluated the TPC of araçá-boi and found mean values of 184.05 mg_GAE/100 g in ethanol extracts, quite below the lowest value found in this study. The IC₅₀ found by the same authors was 0.69 μg/mL, also significantly lower than our results. In our study, a strong correlation was found between IC₅₀ and TPC (Pearson correlation coefficient = 0.86).

The influence of the solvent on the extraction of phenolic compounds and consequently the bioactivity of the extracts was demonstrated in some studies [22, 23]. According to the literature, various solvents are often used for antioxidant activity investigation, such as methanol, ethanol, water, and acetone. Water has a disadvantage over ethanol as it requires a freeze-drying step following extraction, while the use of methanol and acetone is limited due to toxicity issues [24].

A combination of methods will describe the antioxidant properties of the product in further detail. Nevertheless, it should be noted that the comparison between results from different studies is somewhat complex since the characteristics of the fruits tested are different, as well as the conditions of cultivation and preparation.

The highest antioxidant activity and phenolic compound content were obtained at 50°C and 2.0 m/s, which were then selected as the optimal drying condition.

3.3. GC-MS Analyses

The GC-MS analysis of the ethanolic extracts of red araçá fruits (Table 4) showed the existence of various compounds with different chemical structures (terpene, alcohol, ester, ketones, and aldehyde classes).

According to Strehmel et al. [25], the limits of comparison for IR vary according to the complexity of the matrices analyzed, and variations of up to 30 units are accepted for biological matrices. Compounds with highest abundance were found in all extracts, but their relative amount varied. Overall, the major compounds were caryophyllene (9.85–7.49%), 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (pyranone) (7.23–2.07%), selina-3,7(11)-diene (4.14–1.98%), γ-terpinene (3.10–0.06%), γ-cadinene (2.34–1.78%), and α-salinene (2.53–1.86%). The relative concentration of these compounds did not exhibit any trend when the drying temperature or the air velocity was varied ().

Furfural and HMF contents were significantly () affected by both drying temperature and air velocity. The drying temperature and the interaction between the variables had a negative effect on these responses, while air velocity had a positive effect.

Red araçá fruit extracts contain a high number of active compounds (Table 4). The compound 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) is a well-known sugar degradation product, arising from 1-deoxy-d-erythro-hexo-2,3-diulose under neutral pH conditions, that exists in various thermally processed foods such as heated vegetables, meat, caramel sweets, and bread crust [26].

Other sugar derivative compounds in red araçá fruit extracts are 5-hydroxymethylfurfural (HMF) and furfural, which are formed as a response to extended periods of storage or severe heat treatment. According to Chambel et al. [27], temperatures ≥50°C cause HMF degeneration, which may explain the fact that run 2 (60°C and 1.5 m/s), run 4 (60°C and 2.5 m/s), and run 5 (50°C and 2.0 m/s) presented lower HMF concentration than run 3 (40°C and 2.5 m/s) and run 1 (40°C and 1.5 m/s). As these unwanted compounds may threaten human health due to their presumably carcinogenic effects [28], it is recommendable to dry araçá fruits at temperatures of 50°C or higher to promote the degradation of HMF.

Some metabolites have already been identified in red araçá extracts by NMR spectroscopy: triolein, β-sitosterol, ursolic acid, oleanolic acid, 2α-hydroxyursolic acid, 2α-hydroxyoleanolic acid, citric acid, and a mixture of citrate esters (1,2,3-propanetricarboxylic acid-2-hydroxy-1-methyl ester and 1,2,3-propanetricarboxylic acid-2-hydroxy-2-methyl ester). Caryophyllene and its epoxide are well-known volatile constituents of red araçá [8]. As red araçá extracts are important sources of these compounds, their ethanolic extracts have a good market potential.

4. Conclusions

Heat and mass transfer equations were used in the modeling and simulation of the thin-layer convective drying process of red araçá slices. The effective mass diffusivity ranged from 8.542 × 10⁻⁸ to 13.44 × 10⁻⁸ m²/min. For all simulated drying conditions, the coefficient of determination was greater than 97%. Under the conditions studied, the increase in air velocity did not promote acceleration of the drying rate. Thus, conducting the process at 1.5 m/s is sufficient to guarantee a low resistance to mass transfer on the surface of araçá slices and reduce the energy demand of the process.

Fruits dried at 50°C and 2.0 m/s had the highest antioxidant activity as determined by the DPPH method (IC₅₀ = 1265.21 μg_araçá/mL) and the highest TAA (1916.10 mg_{ascorbic acid}/100 g_araçá) as measured by the phosphomolybdenum reduction method. Extracts from fruits dried at 40°C and 1.5 m/s had the highest phenolic content (556.28 mg_GAE/100 g_araçá), which was very close to the value (542.68 mg_GAE/100 g_araçá) determined for mild drying conditions (50°C and 2.0 m/s). Although araçá fruit extracts obtained under different drying conditions presented similar chemical composition, the combination of low temperature with high air velocity seemed to increase the contents of HMF and furfural. The most abundant substances found under these conditions were caryophyllene, 2,3-dihydro-3.5-dihydroxy-6-methyl-4H-pyran-4-one (pyranone), selina-3,7(11)-diene, γ-terpinene, γ-cadinene, and β-salinene. Therefore, drying at 50°C and 2.0 m/s is the best condition (within the range studied) for the maintenance of phenolic compounds and other compounds with antioxidant activity in araçá extracts.

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors have declared no conflicts of interest.

Acknowledgments

The authors would like to thank the CNPq (Brazil’s National Council for Scientific and Technological Development) and CAPES (National Council for the Improvement of Higher Education) for the financial support in the form the scholarships conceded.

References

A. M. F. Drehmer and C. V. T. do Amarante, “Conservação pós-colheita de frutos de araçá-vermelho em função do estádio de maturação e temperatura de armazenamento,” Revista Brasileira de Fruticultura, vol. 30, no. 2, pp. 322–326, 2008.
View at: Publisher Site | Google Scholar
H. Liu, W. Jiang, J. Cao, and L. Ma, “Evaluation of antioxidant properties of extractable and nonextractable polyphenols in peel and flesh tissue of different peach varieties,” Journal of Food Processing and Preservation, vol. 42, no. 6, Article ID e13624, 2018.
View at: Publisher Site | Google Scholar
L. Xia, C. Xu, K. Huang, J. Lu, and Y. Zhang, “Evaluation of phenolic compounds, antioxidant and antiproliferative activities of 31 grape cultivars with different genotypes,” Journal of Food Biochemistry, Article ID e12626, 2018, In press.
View at: Publisher Site | Google Scholar
M. Radojković, M. M. Moreira, C. Soares et al., “Microwave-assisted extraction of phenolic compounds from Morus nigra leaves: optimization and characterization of the antioxidant activity and phenolic composition,” Journal of Chemical Technology and Biotechnology, vol. 93, no. 6, pp. 1684–1693, 2018.
View at: Publisher Site | Google Scholar
J. A. Pino, R. Marbot, and C. Vázquez, “Characterization of volatiles in strawberry guava (psidium cattleianumSabine) fruit,” Journal of Agricultural and Food Chemistry, vol. 49, no. 12, pp. 5883–5887, 2001.
View at: Publisher Site | Google Scholar
A. Luximon-Ramma, T. Bahorun, and A. Crozier, “Antioxidant actions and phenolic and vitamin C contents of common Mauritian exotic fruits,” Journal of the Science of Food and Agriculture, vol. 83, no. 5, pp. 496–502, 2003.
View at: Publisher Site | Google Scholar
A. L. Medina, L. I. R. Haas, F. C. Chaves et al., “Araçá (Psidium cattleianum Sabine) fruit extracts with antioxidant and antimicrobial activities and antiproliferative effect on human cancer cells,” Food Chemistry, vol. 128, no. 4, pp. 916–922, 2011.
View at: Publisher Site | Google Scholar
K. P. McCook-Russell, M. G. Nair, P. C. Facey, and C. S. Bowen-Forbes, “Nutritional and nutraceutical comparison of Jamaican Psidium cattleianum (strawberry guava) and Psidium guajava (common guava) fruits,” Food Chemistry, vol. 134, no. 2, pp. 1069–1073, 2012.
View at: Publisher Site | Google Scholar
C. C. Denardin, G. E. Hirsch, R. F. da Rocha et al., “Antioxidant capacity and bioactive compounds of four Brazilian native fruits,” Journal of Food and Drug Analysis, vol. 23, no. 3, pp. 387–398, 2015.
View at: Publisher Site | Google Scholar
G. Bianchi and R. Lo Scalzo, “Characterization of hot pepper spice phytochemicals, taste compounds content and volatile profiles in relation to the drying temperature,” Journal of Food Biochemistry, vol. 42, no. 6, Article ID e12675, 2018.
View at: Publisher Site | Google Scholar
D. J. Pimentel-González, U. A. Basilio-Cortes, A. D. Hernández-Fuentes et al., “Effect of thermal processing on antibacterial activity of multifloral honeys,” Journal of Food Process Engineering, vol. 40, no. 1, Article ID e12279, 2017.
View at: Publisher Site | Google Scholar
A. B. Ribeiro, R. C. Chisté, M. Freitas, A. F. da Silva, J. V. Visentainer, and E. Fernandes, “Psidium cattleianum fruit extracts are efficient in vitro scavengers of physiologically relevant reactive oxygen and nitrogen species,” Food Chemistry, vol. 165, pp. 140–148, 2014.
View at: Publisher Site | Google Scholar
L. L. Mensor, F. S. Menezes, G. G. Leitão et al., “Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method,” Phytotherapy Research, vol. 15, no. 2, pp. 127–130, 2001.
View at: Publisher Site | Google Scholar
P. Prieto, M. Pineda, and M. Aguilar, “Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E,” Analytical Biochemistry, vol. 269, no. 2, pp. 337–341, 1999.
View at: Publisher Site | Google Scholar
V. L. Singleton and J. A. Rossi, “Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents,” American Journal of Enology and Viticulture, vol. 16, pp. 144–158, 1965.
View at: Google Scholar
F. A. N. Fernandes, F. E. Linhares, and S. Rodrigues, “Ultrasound as pre-treatment for drying of pineapple,” Ultrasonics Sonochemistry, vol. 15, no. 6, pp. 1049–1054, 2008.
View at: Publisher Site | Google Scholar
U. Şahin and H. K. Öztürk, “Effects of pulsed vacuum osmotic dehydration (PVOD) on drying kinetics of figs (Ficus carica L),” Innovative Food Science and Emerging Technologies, vol. 36, pp. 104–111, 2016.
View at: Publisher Site | Google Scholar
T. Vashisth, R. K. Singh, and R. B. Pegg, “Effects of drying on the phenolics content and antioxidant activity of muscadine pomace,” LWT-Food Science and Technology, vol. 44, no. 7, pp. 1649–1657, 2011.
View at: Publisher Site | Google Scholar
D. L. Daniel, B. E. B. Huerta, I. A. Sosa, and M. G. V. Mendoza, “Effect of fixed bed drying on the retention of phenolic compounds, anthocyanins and antioxidant activity of roselle (Hibiscus sabdariffa L.),” Industrial Crops and Products, vol. 40, pp. 268–276, 2012.
View at: Publisher Site | Google Scholar
R. M. Filho, M. R. M. Wolf, and L. G. S. Vasconcelos, “Developement of suitable optimal operating control strategy for large scale dryers,” Computers and Chemical Engineering, vol. 21, pp. S589–S594, 1997.
View at: Publisher Site | Google Scholar
I. A. Neri-Numa, L. B. Carvalho-Silva, J. P. Morales et al., “Evaluation of the antioxidant, antiproliferative and antimutagenic potential of araçá-boi fruit (Eugenia stipitata Mc Vaugh-Myrtaceae) of the Brazilian Amazon Forest,” Food Research International, vol. 50, no. 1, pp. 70–76, 2013.
View at: Publisher Site | Google Scholar
G. Rusak, D. Komes, S. Likić, D. Horžić, and M. Kovač, “Phenolic content and antioxidative capacity of green and white tea extracts depending on extraction conditions and the solvent used,” Food Chemistry, vol. 110, no. 4, pp. 852–858, 2008.
View at: Publisher Site | Google Scholar
B. Zhao and C. A. Hall, “Composition and antioxidant activity of raisin extracts obtained from various solvents,” Food Chemistry, vol. 108, no. 2, pp. 511–518, 2008.
View at: Publisher Site | Google Scholar
M. N. Alam, N. J. Bristi, and M. Rafiquzzaman, “Review on in vivo and in vitro methods evaluation of antioxidant activity,” Saudi Pharmaceutical Journal, vol. 21, no. 2, pp. 143–152, 2013.
View at: Publisher Site | Google Scholar
N. Strehmel, J. Hummel, A. Erban, K. Strassburg, and J. Kopka, “Retention index thresholds for compound matching in GC-MS metabolite profiling,” Journal of Chromatography B, vol. 871, no. 2, pp. 182–190, 2008.
View at: Publisher Site | Google Scholar
F. Ledl, W. Schnell, and T. Severin, “Nachweis von 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-on in Lebensmitteln,” Zeitschrift für Lebensmitteluntersuchung und-Forschung, vol. 160, no. 4, pp. 367–370, 1976.
View at: Publisher Site | Google Scholar
P. Chambel, M. B. Oliveira, P. B. Andrade, J. O. Fernandes, R. M. Seabra, and M. A. Ferreira, “Identification of 5,5′-oxy-dimethylene-bis(2-furaldehyde) by thermal decomposition of 5-hydroxymethyl-2-furfuraldehyde,” Food Chemistry, vol. 63, no. 4, pp. 473–477, 1998.
View at: Publisher Site | Google Scholar
E. Capuano and V. Fogliano, “Acrylamide and 5-hydroxymethylfurfural (HMF): a review on metabolism, toxicity, occurrence in food and mitigation strategies,” LWT-Food Science and Technology, vol. 44, no. 4, pp. 793–810, 2011.
View at: Publisher Site | Google Scholar

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Copyright © 2019 Michele C. M. Bombardelli et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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