Current Status and Future Development of Cell Transplantation Therapy for Periodontal Tissue Regeneration
Figure 4
Tumorigenic and regenerative properties of human PDL cell sheets. (a)–(c) Tumorigenesis in vivo. Immunodeficient mice were injected with medium only (negative control), human PDL cells, or HeLa S3 cells (positive control). Mice injected with human PDL cells or the medium control did not form tumour-like tissue (a, b), whereas mice injected with HeLa S3 cells exhibited tumour formation (c). (d)–(f) Histology and immunohistochemistry of implanted human PDL cell sheets with dentin blocks. Newly formed cementum- (arrow) and PDL-like tissue (arrowheads) were observed around the dentin blocks (d, e). Immunostaining with an antibody against human vimentin revealed the presence of human PDL cells around the dentin block (f). Scale bars: 500 μm in (a) and (b); 1 mm in (c); 50 μm in (d)–(f). Modified from [25] with permission.
