Effect of Vitronectin Bound to Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-3 on Porcine Enamel Organ-Derived Epithelial Cells
Figure 4
Immunofluorescence analysis in enamel organ-derived epithelial (EOE) cells. (a) Immunofluorescence showed that EOE cells were positive for amelogenin in the collagen type I-coated dishes. (b) Merged image to (a). Amelogenin staining in combination with DAPI to stain DNA (blue). (c) EOE cells were positive for amelogenin by immunofluorescence in the insulin-like growth factor-I/insulin-like growth factor binding protein-3/vitronectin complex-coated dishes. The staining of amelogenin in the EOE cells was more intense than that in the collagen type I-coated dishes. (d) Merged image to (c). Amelogenin staining in combination with DAPI to stain DNA (blue). Scale bars: 50 μm length (a–d). (e) The high magnification view of (d). EOE cells cultured on in the insulin-like growth factor-I/insulin-like growth factor binding protein-3/vitronectin complex-coated dishes expressed strongly amelogenin.
