Peri-Implant Crestal Bone Loss: A Putative Mechanism

Figure 6

Expression of pro-inflammatory cytokine genes in rat stromal bone marrow cells. Gene expression for IL-1α, IL-1β, TNF-α, IL-6 and GAPDH in rSBMCs cultures in the presence and absence of LPS. The LPS concentrations used were 0.1 μg/mL and 1 μg/mL. RNA was isolated at 6, 24, 48 and 96 hours. RT-PCR was carried out at 30 cycles for GAPDH, IL-1α, IL-1β, and TNF-α, and 25 cycles for IL-6.