Research Article
Peri-Implant Crestal Bone Loss: A Putative Mechanism
Table 1
Specific primers for the reverse transcriptase polymerase chain reaction (RT-PCR) and the predicted sizes of the PCR products (bp).
(a) Specific primers for mouse |
| Specificity | Primer | Detection | Product size (bp) |
| IL-1alpha | Sense | TGCCACCAAAGAACAAAGTCG | 599 | Antisense | CCCACTGAGGTAGGAAAGATGTAGC |
| IL-1beta | Sense | TCTTTGAAGTTGACGGACCCC | 269 | Antisense | CCAGCAGGTTATCATCATCATCCC |
| TNF-alpha | Sense | ACCGTCAGCCGATTTGCTATC | 288 | Antisense | TCAGAGTAAAGGGGTCAGAGTGGG |
| IL-6 | Sense | GAGCCCACCAAGAACGATAG | 229 | Antisense | TCCACGATTTCCCAGAGAAC |
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(b) Specific primers for rat |
| Specificity | Primer | Detection | Product size (bp) |
| IL-1alpha | Sense | AGGTTCCACGTTTCCTCCTT | 233 | Antisense | GCCTCCAGGTCATCTTCAGT |
| IL-1beta | Sense | CAACAAAAATGCCTCGTGC | 395 | Antisense | AAGTCAACTATGTCCCGAC |
| TNF-alpha | Sense | TACTGAACTTCGGGGTGATCG | 292 | Antisense | CCTTGTCCCTTGAAGAGAACC |
| IL-6 | Sense | TGTGCAATGGCAATTCTGAT | 156 | Antisense | GGAACTCCAGAAGACCAGAGC |
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(c) Specific primers for human |
| Specificity | Primer | Detection | Product size (bp) |
| IL-1alpha | Sense | TTCATTGGCGTTTGAGTCAG | 163 | Antisense | GGAGTGGGCCATAGCTTACA |
| IL-1beta | Sense | GCATCCAGCTACGAATCTCC | 193 | Antisense | TCGTTATCCCATGTGTCGAA |
| TNF-alpha | Sense | ACAAGCCTGTAGCCCATGTT | 265 | Antisense | TTGATGGCAGAGAGGAGGTT |
| IL-6 | Sense | GCTATGAACTCCTTCTCCACAAGC | 264 | Antisense | TTCTGCCAGTGCCTCTTTGC |
| RANK | Sense | ATGCGGTTTGCAGTTCTTCT | 384 | Antisense | CGTAGGGACCACCTCCTACA |
| RANKL | Sense | AGAGCGCAGATGGATCCTAA | 180 | Antisense | TTCCTTTTGCACAGCTCCTT |
| TRL 2 | Sense | TTAGCAACAGTGACCTACAGAG | 503 | Antisense | CAAATCAGTATCTCGCAGTTCC |
| TRL 4 | Sense | TGGATACGTTTCCTTATAAG | 507 | Antisense | GAAATGGAGGCACCCCTTC |
| CD 14 | Sense | CAACTTCTCCGAACCTCAGC | 271 | Antisense | TAGGTCCTCGAGCGTCAGTT |
| MD-2 | Sense | GCACATTTTCTACATTCC | 157 | Antisense | CACAGTCTCTCCCTTCAG |
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(d) Specific primers for House keeping gene |
| Specificity | Primer | Detection | Product size (bp) |
| GAPDH | Sense | ACCACAGTCCATGCCATCAC | 452 | Antisense | TCCACCACCCTGTTGCTGTA |
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