Effect of S-PRG Eluate on Biofilm Formation and Enzyme Activity of Oral Bacteria
Figure 3
Gelatinase spot assay. Different amounts of S-PRG eluate were added to the reaction mixture. P.-gingivalis-sonicated extract was then added and the mixture was serially diluted with distilled water. A 15 μL spot of each mixture was placed onto gelatin-coated X-ray film and incubated at 37°C for 2 h in a humidified atmosphere before being washed with tap water. Removal of the gelatin surface beneath the spotted area revealed the base color of the film and indicated the presence of gelatinase activity. (a) Photograph of X-ray film after assay. (b) Results of gelatinase spot assay.
