Research Article

Effect of Dentin Biomodification Using Naturally Derived Collagen Cross-Linkers: One-Year Bond Strength Study

Table 2

Bond strength (MPa) of demineralized dentin treated (standard deviation) with different dentin biomodification agents in different application times and storage periods for Single Bond Plus (SB) and One Step Plus (OS). One-way ANOVA values for different statistical groups.

Adhesive systemVariablesμBS (MPa)
Storage time
24 hours3 months6 months12 months

SBGSE
 10 min65.96 (12.36)Aa64.17 (16.72)Aa64.17 (16.16)Aa57.15 (15.29)Aa0.185
CSE/ET
 10 min56.95 (14.40)Aab55.90 (14.11)Aab52.88 (11.46)Ab54.04 (17.12)Aa0.753
CSE/AC
 10 min49.97 (11.91)Ab51.75 (16.82)Ab49.98 (13.97)Ab48.22 (19.46)Aa0.890
Control
 10 min54.51 (20.03)Ab52.74 (16.31)Aab49.16 (13.88)Ab51.68 (19.42)Aa0.759
0.0030.0310.0010.347

OSGSE
 10 min60.93 (8.79)Aa53.57 (12.4)Ba50.41 (10.14)Ba42.62 (7.46)Cab0.000
CSE/ET
 10 min60.28 (12.86)Aa55.18 (11.26)Aa47.01 (8.25)Bab44.6 (10.95)Ba0.000
CSE/AC
 10 min54.44 (11.01)Aab55.76 (9.70)Aa48.90 (16.50)ABab41.30 (6.83)Bab0.000
Control
 10 min50.39 (18.17)Ab49.66 (9.38)Aa39.11 (12.71)Bb36.85 (10.41)Bb0.000
0.0160.1870.0110.036

GSE: grape seed extract; CSE: cocoa seed extract; ET: ethanol; AC: acetone; : probability value. Different lower and upper case letters show statistical differences among rows and columns, respectively. Note: adhesive systems were not compared with each other.