Assessment of Cytokine Release against Oral Mucosal Cell Line Culture (TR146) Stimulated by Neutrophil Elastase Associated with Behcet’s Disease

Table 2

Detection of IL-1β following the treatment of the OMM with NE after 24- and 4-hour incubation.

Parameters	NE 10 nM	NE 50 nM	NE 100 nM	NE 200 nM	Negative control (saline)	Positive control (SLS 1%)
IL-1β (pg./ml) 24 hours of incubation	—	—	1.31^d/d† ± 0.06	1.33^e/e† ± 0.02	0.99 ± 0.07	± 0.21	a/a†
IL-1β (pg./ml) 4 hours of incubation	46.02^ns/b† ± 4.45	2.42^ns/c† ± 0.23	2.42^dgi/d†g†i† ± 0.21	1.92 ^e† ± 0.08	2.53 ± 0.25	± 4.45	a/a†

; ; . ns = nonsignificant, a/a† = compared with the negative control/compared with the positive control, b/b† = compared the negative control with 10 nM NE/compared the positive control with 10 nM NE, c/c† = compared the negative control with 50 nM NE/compared the positive control with 50 nM NE, d/d† = compared the negative control with 100 nM NE/compared the positive control with 100 nM NE, e/e† = compared the negative control with 200 nM NE/compared the positive control with 200 nM NE, g/g† = compared 10 nM NE with 100 nM NE (negative control group)/compared 10 nM NE with 100 nM NE (positive control group), and i/i† = compared 50 nM NE with 100 nM NE (negative control group)/compared 50 nM NE with 100 nM NE (positive control group).