Prolactin and Male Fertility: The Long and Short Feedback Regulation

Table 1

Release of Prolactin by different pituitary constructs in vitro.

System	Whole pituitary		Whole pituitary		Pituitary-hypothalamus
			Plus hypothalamus		complex
Incubation(h)	Total^a	Per h^b	Total^a	Per h^b	Total^a	Per h^b
2	371.3±42.9	185.6±21.5	281.4±22.9	140.7±11.5	227.9±28.9^b	113.9±14.4
4	360.2±34.6	90±8.7^**	294.9±31.1	73.7±7.8^*	345.4±21.9^**	86.4±5.5
6	437.8±56.6	72.5±9.4^*	401.9±24.3^**	67±4.1^*	270.4±30.7^c	45±5.1^*

Pituitary constructs were incubated in DMEM at 37^°C for 4 hours, in replicates of 10, at each time interval. Prolactin was assayed in spent medium by RIA. Values are expressed as mean ± SEM. ^a ng Prl (NIAMDD-RP-2); ^*𝑃<.001, ^**𝑃<.01, with respect to levels at 2 hours; ^b 𝑃<.02, ^c 𝑃<.05, with respect to corresponding pituitary levels [27].