Review Article

Prolactin and Male Fertility: The Long and Short Feedback Regulation

Table 2

Effects of reserpine on prolactin secretion by different pituitary constructs in response to dopamine agonists and antagonists in vitro.

AdditionsControlReserpine 1.5 mg/kg/d
In 25% Ascorbic acidin 25% ascorbic acid
(in vitro)PIP-NPHcPIP-NPHc

Ascorbic acid429 ± 14.8 702 ± 8.54 c 316 ± 7.59 126 ± 1.9 b 126 ± 3.8 b 167 ± 4.4 bc
(0.05%)
Dopamine 344 ± 12.34219 ± 11.39 a 124 ± 2.85 a 50 ± 1.27 ab 71 ± 3.8 ab 59 ± 1.9 ab
1 0 7  M
Bromocriptine 317 ± 10.12339 ± 5.38 a 161 ± 5.06 a 46 ± 2.53 ab 56 ± 3.8 ab 50 ± 1.9 ab
1 0 7  M
Apomorphine 364 ± 5.06 108 ± 5.38 a 108 ± 11.71 a 40 ± 1.27 ab 60 ± 2.53 ab 30 ± 1.27 b
8 × 1 0 7  M
Perphenazine 393 ± 10.12694 ± 3.8 c 466 ± 5.7*140 ± 3.8140 ± 6.96330 ± 5.7*
5 × 1 0 7  M

Reserpine was dissolved in 25% ascorbic acid and administered s.c to adult male rats for 46 hours. Control group rats received vehicle alone. Different pituitary constructs were dissected out in DMEM and incubated at 37°C for 4 hours, in replicates of 10, at each time interval. Prolactin was assayed in spent medium by RIA. Values are expressed as mean (ng NIDDK-RAT-PRL-RP-3-ml incubation medium) ± SEM. a significant difference with respect to 0.05% ascorbic acid ( 𝑃 . 0 5 ). b significant difference with respect to 25% ascorbic acid control group ( 𝑃 . 0 5 ). c significant difference with respect to PI and PHc of the same group ( 𝑃 . 0 5 ). *significant difference with respect to PI and P-N of the same group ( 𝑃 . 0 5 ) [33].