The Involvement of Ser1898 of the Human L-Type Calcium Channel in Evoked Secretion
Figure 3
The kinetics of spike and foot parameters elicited in GFP- and human-wt 1.2-(Hu/wt-) infected cells are similar. (a) Amperometric currents representing single-vesicle events were triggered by a 10 sec pulse of K60 in cells infected with pSFV containing GFP or Hu/wt in the presence of 5 M nifedipine. (b) The kinetic properties of a single amperometric event: peak amplitude, half-width, 50–90% rise time, and integrated spike (Q, grayarea). Foot signal: foot-width, foot-amplitude, and integrated foot (Q, whitearea). (c) Cumulative distribution plots of spike number and analysis of peak amplitude induced in GFP- or Hu/wt-infected cell; (inset), mean values for peak amplitude , events per data point, respectively. (d) Cumulative distribution plot of spike number and analysis of 50–90% rise time detected in GFP- and Hu/wt-infected cells. Data plotted in a similar fashion as in (c); mean values for 50–90% rise time (inset). (e) Cumulative distribution plot of spike number and analysis of half-width in GFP- or Hu/wt-infected cells (inset), mean values for spike half-width (f) Cumulative distribution plot of spike number and analysis of mean charge in GFP- or Hu/wt infected cells; (inset), mean values for spike mean charge. (g) Cumulative distribution plot of foot number and analysis of foot amplitude in GFP- or Hu/wt-infected cells (inset), mean values for foot amplitude. (h) Cumulative distribution plot of foot number and analysis of foot width in GFP- or Hu/wt-infected cells (inset), mean values for foot width *.
