The Involvement of Ser1898 of the Human L-Type Calcium Channel in Evoked Secretion
Figure 4
Spike and foot parameters of Hu/wt are not affected by the S1898A mutation. Amperometric current triggered in the presence of 5 M nifedipine by a 10 sec pulse of K60 as indicated by the arrow. (a) Representative single amperometric events triggered in cells infected with Hu/wt subunit () or (b) Hu/S1898A (). (c) Cumulative distribution plot of spike number and analysis of peak amplitude induced in Hu/wt- and Hu/S1898A-infected cells (inset) mean values for peak amplitude , events per data point, respectively. (d) Cumulative distribution plot of spike number and analysis of 50–90% rise time detected in Hu/wt- and Hu/S1898A-infected cells. Data plotted similar fashion as in panel (c), mean values for 50–90% rise time (inset). (e) Cumulative distribution plot of spike number and analysis of half-width of Hu/wt- and Hu/S1898A-infected cells; mean values for spike half-width (inset). (f) Cumulative distribution plot of spike number and analysis of mean charge in Hu/wt- and Hu/S1898A-infected cells; mean values for spike mean charge (inset). (g) Cumulative distribution plot of foot number and analysis of foot amplitudes in Hu/wt- and Hu/S1898A-infected cells; mean values for foot amplitude (inset). (h) Cumulative distribution plot of foot number and analysis of foot width in Hu/wt- and Hu/S1898A-infected cells; mean values for foot width (inset).
