International Journal of Endocrinology / 2011 / Article / Fig 4

Research Article

Androgen Receptor Regulates Transcription of the ZEB1 Transcription Factor

Figure 4

Two functional AREs are present in the tcf8 5′-flanking region. (a) Two well-conserved AREs were identified in tcf8 at −944 and −140 (white boxes) relative to the translation start site. Plasmids were constructed in which each of the putative AREs was mutated individually or both were mutated (black boxes). −36 is the putative transcription start site and is numbered relative to the translation start site. (b). The plasmids depicted in (a) were transfected into PC-3/AR cells with increasing concentrations of DHT as indicated. The cells were harvested after 24 hrs and the β-galactosidase activity in the lysates was determined. relative to no DHT. with 2-3 replicates per experiment.

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