International Journal of Endocrinology / 2011 / Article / Fig 5

Research Article

Androgen Receptor Regulates Transcription of the ZEB1 Transcription Factor

Figure 5

The AREs in tcf8 bind AR. PC-3/AR cells were treated with 5 nM DHT for 24 hrs, and nuclear protein extracts prepared. Oligomers corresponding to the regions of DNA containing (a) the −944 ARE and (b) the −140 ARE were radiolabeled and used as probes. Both GMSAs were run in parallel, with only the ARE probe differing. Ten μg of nuclear protein was used in each lane except for Lane 1, which contained only the radioactive oligomer. Lane 2: plus nuclear extract. Lane 3: plus 50x cold wild-type (WT) self-competitor. Lane 4: plus 50x cold self-competitor with a mutated (MT) ARE. Lane 5: plus 50x cold WT consensus ARE. Lane 6: plus 50x cold MT consensus ARE. Lane 7: plus an AR antibody. The arrow indicates the band representing the AR/DNA complexes supershifted by the antibody.

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