Androgen Receptor Regulates Transcription of the ZEB1 Transcription Factor

Figure 6

DHT induces the tcf8 promoter plasmid in a dose-dependent manner in LNCaP cell line derivatives. The LNCaP (a), C4 (b), C4-2 (c), and C4-2B (d) cell lines were transfected with the pBlueZEB974 promoter plasmid and then cultured with increasing DHT as indicated. The cells were harvested after 24 hrs and the β-galactosidase activity in the lysates was determined. (e) C4-2 cells were transfected with the wild type pBlueZEB974 plasmid or with the plasmids containing one or both AREs mutated. Data are plotted relative to the average with no DHT. ^∗𝑃<0.0001, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.05.

(a)

(b)

(c)

(d)

(e)