International Journal of Endocrinology / 2011 / Article / Fig 7

Research Article

Androgen Receptor Regulates Transcription of the ZEB1 Transcription Factor

Figure 7

Endogenous tcf8 is not induced in the LNCaP cell lines. The four LNCaP-derived cell lines were each divided into two groups and were incubated with or without 5 nM DHT for 24 hrs. (a) For one group, RNA was isolated and subjected to qPCR for ZEB1 and GAPDH mRNA. ZEB1 mRNA levels were normalized to GAPDH. (b) For the second group, the cells were transfected with pBlueZEB974 at T = O, and β-galactosidase activity was determined at 24 hrs. compared to no DHT.

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