Research Article
Androgen Receptor Regulates Transcription of the ZEB1 Transcription Factor
Table 1
The oligomer sequences used for qPCR, mutagenesis, and GMSA.
| | Oligomer | Oligomer Sequence | Use |
| | ZEB1 (F) | 5′-TCCTGAGGCACCTGAAGAGG-3′ | PCR | | ZEB1 (R) | 5′-CAGAGAGGTAAAGCGTTTATAGCC-3′ | PCR | | RPL32 (F) | 5′-CCATCTCCTTCTCGGCATCAT G-3′ | PCR | | RPL32 (R) | 5′-GGT TTCCGCCAGTTACGCTTA-3′ | PCR | | GAPDH (F) | 5′- GACCCCTTCATTGACCTCAACTACATG-3′ | PCR | | GAPDH (R) | 5′-CTCCTGGAAGATGGTGATGGG-3′ | PCR | | MT ARE (−944 to −930) | 5′GAGCCTCTAGGTGTgAattcGGTGAgaaCGcAAAGCCGGGAGTGT3’ | Mutation | | MT ARE (−140 to −126) | 5′GAGCCTCTAGGTGTgAattcGGTGAgaaCGcAAAGCCGGGAGTGT3’ | Mutation | | WT ARE (−944 to −930) | 5′-agcttCCTAGGATCCCACGGTTCTACGCGAGGAAGAGa-3′ | GMSA | | MT ARE (−944 to −930) | 5′-agcttCCTAGtcTCCCACGtaTCTACGCGAGtcAGAGa-3′ | GMSA | | WT ARE (−140 to −126) | 5′-agcttTGTAAGGAAGGTGATGTCGTAAAGCCa-3′ | GMSA | | MT ARE (−140 to−126) | 5′-agcttTGTgAattcGGTGAgaaCGcAAAGCCa-3′ | GMSA | | WT Consensus ARE | 5′-agcttCTAGAAGTCTGGTACAGGGTGTTCTTTTTGCAa-3′ | GMSA | | MT Consensus ARE | 5′-agcttCTAGAAGTCTGcaACAGGGTcaTCTTTTTGCAa-3′ | GMSA |
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For the site-directed mutagenesis and GMSA oligomers, only the top strand is shown. Small letters indicate mutations or restriction enzyme sites.
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