Nerium oleander Distillate Improves Fat and Glucose Metabolism in High-Fat Diet-Fed Streptozotocin-Induced Diabetic Rats

Table 5

Primer sequences of PPAR-α, PPAR-β, PPAR-γ, β-Actin.

	Primer sequences	PCR product size-bp	At (°C)
PPAR-α	5′cccaatggttgctgattaca-3; 5′ggacgcaggctctactttga-3	580	56
PPAR-β	5′cccaatggttgctgattaca-3; 5′ggacgcaggctctactttga-3	202	56
PPAR-γ	5′tgcggactaccagtacttaggg-3; 5′ggaagctggagagagggtgt-3	210	59
β-Actin	5′ccctcatagatgggcacagt-3	4477	50
	5′gtagccatccaggctgtgtt-3
PCR Protocol	92°C 1 min 60°C 1 min 72°C 1 min