TY - JOUR
A2 - Fröhlich, Eleonore
AU - Ahn, Byeong-Cheol
AU - Lee, Won Kee
AU - Jeong, Shin Young
AU - Lee, Sang-Woo
AU - Lee, Jaetae
PY - 2013
DA - 2013/03/31
TI - Estimation of True Serum Thyroglobulin Concentration Using Simultaneous Measurement of Serum Antithyroglobulin Antibody
SP - 210639
VL - 2013
AB - We investigated the analytical interference of antithyroglobulin antibody (TgAb) to thyroglobulin (Tg) measurement and tried to convert measured Tg concentration to true Tg concentration using a mathematical equation which includes a concentration of TgAb. Methods. Tg was measured by immunoradiometric assay and TgAb by radioimmunoassy. Experimental samples were produced by mixing Tg and TgAb standard solutions or mixing patients’ serum with high Tg or high TgAb. Mathematical equations for prediction of expected Tg concentration with measured Tg and TgAb concentrations were deduced. The Tg concentration calculated using the equations was compared with the expected Tg concentration. Results. Measured Tg concentrations of samples having high TgAb were significantly lower than their expected Tg concentration. Magnitude of TgAb interference with the Tg assay showed a positive correlation with concentration of TgAb. Mathematical equations for estimation of expected Tg concentration using measured Tg and TgAb concentrations were successfully deduced and the calculated Tg concentration showed excellent correlation with expected Tg concentration. Conclusions. A mathematic equation for estimation of true Tg concentration using measured Tg and TgAb concentration was deduced. Tg concentration calculated by use of the equation might be more valuable than measured Tg concentration in patients with differentiated thyroid cancer.
SN - 1687-8337
UR - https://doi.org/10.1155/2013/210639
DO - 10.1155/2013/210639
JF - International Journal of Endocrinology
PB - Hindawi Publishing Corporation
KW -
ER -