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International Journal of Endocrinology
Volume 2013 (2013), Article ID 313528, 7 pages
http://dx.doi.org/10.1155/2013/313528
Research Article

The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model

1Department of Nephrology, Bulent Ecevit University Medical School, Esenkoy, 67100 Zonguldak, Turkey
2Department of Internal Medicine, Ege University Medical School, 35100 Izmir, Turkey
3Department of Pharmacology, Ege University Medical School, 35100 Izmir, Turkey
4Department of Biochemistry, Ege University Medical School, 35100 Izmir, Turkey
5Department of Pathology, Ege University Medical School, 35100 Izmir, Turkey

Received 5 April 2013; Revised 28 May 2013; Accepted 30 May 2013

Academic Editor: Hulya Taskapan

Copyright © 2013 Ender Hur et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI. Methods. Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1α, 25 (OH)2D3 0.4 mcg/kg/day subcutaneously for 8 days. Blood pressures and 24-hour urine were measured. Blood urea and creatinine levels and urine tubular injury markers were measured. Renal histology was semiquantitatively assessed. Results. Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model. Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group. Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups. Conclusions. Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system.