Sperm Proteomics: Road to Male Fertility and Contraception

Table 2

Proteomic studies on human spermatozoa to access male fertility.

Authors	Proteomic technique	Study outcome
Zhao et al. [75]	MALDI-TOF	Identification of 10 differentially expressed proteins in asthenozoospermic patients compared with those of normozoospermic donors
Martínez-Heredia et al. [40]	MALDI-TOF	Identification of 131 proteins to provide a reference 2D map of mature human sperm spermatozoa
Johnston et al. [76]	LC-MS/MS	Identification of 1760 proteins that comprise human sperm
Domagala et al. [77]	LC-MS	Identification of 35 proteins as sperm immunogenic antigens
Secciani et al. [78]	MS/MS	Identification of protein profiles of capacitated versus ejaculated spermatozoa
Li et al. [20]	MALDI-MS	Establishment of a high-resolution 2D map of human sperm proteins by identifying 16 proteins
Chan et al. [79]	MADLI-TOF	Identification of 12 proteins to address altered protein phosphorylation and aberrant sperm motility
Baker et al. [42]	LC-MS/MS	Identification of 1056 gene products from human sperm populations
Lefièvre et al. [80]	MS/MS	Identification of 240 S-nitrosylated proteins detected to address nitric oxide-induced capacitation
De Mateo et al. [81]	MALDI-TOF	Identification of 101 spots from infertile patients
Bohring et al. [22]	MALDI-MS	Identification of 18 proteins (sperm membrane antigens)

MALDI-TOF: matrix-assisted laser desorption/ionization-time of flight; LC-MS/MS: liquid chromatography-tandem mass spectrometry; LC-MS: liquid chromatography-mass spectrometry; MS/MS: tandem mass spectrometry.