International Journal of Endocrinology / 2013 / Article / Fig 4

Research Article

Effect of Gelam Honey on the Oxidative Stress-Induced Signaling Pathways in Pancreatic Hamster Cells

Figure 4

Effect of quercetin and Gelam honey extract on pAkt (Ser473) expression.Quantitative analysis and representative western blot analysis of pAkt (Ser473) in HIT-T15 cells pretreated with quercetin and honey extract in cells cultured in 20 mM ((a), (c), (e)) and 50 mM ((b), (d), (f)) glucose. A sustained increase in the level of pAkt (ser473) was observed after pretreatment with quercetin and honey extract. Akt inhibitor VIII prevented the expression Akt ser473 phosphorylation induced by quercetin and honey extract. The results were normalized with β actin antibody. Data were presented as the mean ± standard deviation. (e) ; quercetin and honey extract treated compared to the 20 mM glucose alone. (f) ; quercetin and honey extract treated compared to the 50 mM glucose alone.