Omentin-1 Stimulates Human Osteoblast Proliferation through PI3K/Akt Signal Pathway
Omentin-1 activated Akt signal pathway in hOB. (a) Western blot analysis of Akt activation. The hOBs were cultured in serum-free a-MEM for 6 h and then treated with omentin-1 (200 ng/mL) for 5–60 min. The cell lysates were analyzed by western blotting and incubated with antibodies against p-Akt and Akt. Representative results are shown. (b) Either control siRNA or Akt siRNA was transfected into osteoblast. Akt siRNA significantly suppressed the expression of phosphor-Akt (left). Expression of Akt was determined by western blot analysis using an Akt antibody. Representative results are shown (right).