Negative Effects of High Glucose Exposure in Human Gonadotropin-Releasing Hormone Neurons

Figure 1

Gene expression profiling in FNC-B4 cells. Relative mRNA expression of genes relevant for GnRH neuron function was evaluated using quantitative RT-PCR. Data were calculated according to the comparative Ct method, using 18S rRNA subunit as the reference gene for normalization. Measurements were performed in four different cell preparations at different passages (from 5 to 10). No statistical differences were detected among passages, then results were pooled and reported as the mean ± SEM.