Negative Effects of High Glucose Exposure in Human Gonadotropin-Releasing Hormone Neurons
Figure 3
Effect of high glucose on leptin signaling in FNCB4 cells. (a) Quantitative RT-PCR analysis of LEPR mRNA expression in FNC-B4 cells exposed to normal (NG, 5 mM), high (HG, 22 mM), and very high (VHG, 40 mM) glucose concentration or mannitol (M, 22 mM) for 24 hours. (b) Effect of leptin (1 nM, 24 hours) on GnRH mRNA expression in FNC-B4 cells exposed to the different glucose concentrations (NG, HG, and VHG) or mannitol (M, 22 mM). Results were calculated according to the comparative Ct method, using 18S rRNA subunit as the reference gene for normalization and were obtained from at least three separate experiments, each performed in triplicate. Data are reported as mean SEM and are expressed in % of NG. *; ** versus NG.
