Figure 5: ERK signaling pathway and estrogen receptor mediated the antiapoptotic effects of puerarin in hOBs. Cells were incubated with PD98059 (10 μM) and/or ICI 182780 (10 μM) for 3 h prior to treatment with 10−8 M puerarin for 48 h. The bars represented the mean SD ( ). , as compared with the puerarin-treated cells; , as compared with the serum-free treated cells.