Gram-Negative Bacterial Lipopolysaccharide Stimulates Activin A Secretion from Human Amniotic Epithelial Cells
Figure 3
Effect of LPS on BrdU incorporation in AEC. (a) AEC were seeded in 96-well microplates at densities of 1,250, 2,500, 5,000, 10,000, and 20,000 cells per well in 200 μL of DME/Ham’s F12 medium supplemented with 10% FBS, 100 μg/mL streptomycin, and 0.5 μg/mL amphotericin B. Either LPS (10 μg/mL) or vehicle (control) was added to each well. After 96 h of culture, BrdU incorporation was studied. The results from the quadruplicate assay are shown as the mean ± SE (). compared with the control value at each cell density. compared with the control value at each cell density. (b) Fetal membranes were obtained from three patients. AEC from each patient were cultured independently in DME/Ham’s 12 medium supplemented with 10% FBS, 100 μg/mL streptomycin, 0.5 μg/mL amphotericin B, and various concentrations of LPS. After 96 h of culture, BrdU incorporation was studied. Closed circles, closed rectangles, and closed triangles show AEC from each patient. The absorbance at AEC cultured without LPS is shown as 100%. The results from the quadruplicate assay are shown as the mean ± SE (). compared with the control (LPS; 0 ng/mL) value. compared with the control (LPS; 0 ng/mL) value.
