The ETS-Domain Transcription Factor Elk-1 Regulates COX-2 Gene Expression and Inhibits Glucose-Stimulated Insulin Secretion in the Pancreatic β-Cell Line INS-1
Figure 5
Elk-1 inhibited glucose-stimulated insulin secretion in INS-1 cells. INS-1 cells were transfected with Elk-1 expression plasmid pCMV3.0b-Elk-1 or empty vector pCMV3.0b as control for 48 h. Control cells demonstrated a 6.9-fold increase in insulin secretion with 16.7 mM glucose, whereas Elk-1 overexpressing cells had only a 3.6-fold increase. Elk-1 overexpressing group demonstrated a decrease of GSIS to 52% of the control value. Each experiment was done in triplicate and repeated three times. * versus control.
