Research Article

Estrogen Induces Metastatic Potential of Papillary Thyroid Cancer Cells through Estrogen Receptor α and β

Figure 4

E2 induces metastasis via downregulation of E-cadherin and upregulation of vimentin and MMP-9. BCPAP cells were treated with vehicle (DMSO) alone, 10−8 M E2, 10−6 M PPT, or 10−6 M DPN. Whole cell lysates were extracted, and E-cadherin, vimentin, and MMP-9 protein were detected by western blot analysis. β-actin was used as a loading control. * compared with control.