Negative Influence of a Long-Term High-Fat Diet on Murine Bone Architecture
Figure 4
Microstructure of cancellous bone in demineralized paraffin sections. Trabeculae of control mice ((a)–(d)) exhibit a plate-like shape ((a)-(b)) whereas trabeculae of HFD mice ((e)-(f)) show a more rod-like form ((e)-(f)). The trabecular lamellae were interrupted by spaces in HFD mice (arrow, (f)). Collagen-1 immunohistochemistry (col1, (c), (g)) brought out an increase in woven bone (arrow) in (g) HFD mice in comparison to the lamellar trabeculae in (c) control animals. Changes were neither demonstrated in enzyme histochemistry of alkaline phosphatase (arrow, ALP, (d)) nor in tartrate-resistant acidic phosphatase (arrow, TRAP, (h)). Scale bar: 500 μm in (a), (e); 50 μm in (b), (c), (f); 20 μm in (d), (g), (h).
