High Risk First Degree Relatives of Type 1 Diabetics: An Association with Increases in CXCR3+ T Memory Cells Reflecting an Enhanced Activity of Th1 Autoimmune Response
Figure 2
Expression profiles of CXCR3 and CCR4 on circulating CD45RO+ and CD4+ T cells. (a) Cells from lymphocyte gate were selected and (b) population of CD4+/CD45RO+ cells was chosen for chemokine receptor analysis. (c) Representative dot plots of CXCR3 and CCR4 expression on CD4+/CD45RO+ cells from analysis of high risk first degree relatives (hrFDR), low risk first degree relatives (lrFDR), patients with recent-onset type 1 diabetes in insulin requiring state (RT1D-IRS), or healthy control (HC) blood sample following the 4-color staining using corresponding mAbs and flow-cytometric analysis. The results show the percentage of CD45RO+/CD4+ cells expressing CXCR3 and CCR4 in each representative sample.
