Irisin Enhances Osteoblast Differentiation In Vitro

Figure 1

qPCR analysis of FNDC5 in mRNA extracts (a) and western blot analysis of Irisin/FNDC5 in total cell lysates (b) from primary culture of myoblasts obtained from mice subjected to 3 weeks of rest activity or free wheel running activity. 𝑁=8 for each group, repeated in 3 separate experiments. Data is presented as mean ± SEM. ^∗∗𝑃<0.001 and ^∧𝑃<0.05 compared to rest group. Student’s 𝑡-test was used for single comparison.

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