Ghrelin’s Effects on Proinflammatory Cytokine Mediated Apoptosis and Their Impact on β-Cell Functionality

Figure 1

Ghrelin and cytokines’ effects on cell survival and its survival pathways. Trypan blue exclusion test of cell viability (a), MTT assay for cell viability (b), and TUNEL-positive cells quantification to determine ghrelin’s effects on apoptosis-mediated cellular death (c). Cells were incubated for 48 h in the absence or presence of cytokines, ghrelin, or both. (d) shows representative photomicrographs of control, cytokines (CTK), ghrelin (GHR), and cytokines + ghrelin (CTK + GHR), between groups.