Ghrelin’s Effects on Proinflammatory Cytokine Mediated Apoptosis and Their Impact on β-Cell Functionality

Figure 3

Ghrelin and cytokines’ effects on levels of caspases and Bcl-2 protein family members. Immunoblotting was performed to measure relative mean levels of active caspase-8 (a); active caspase-9 (b); BID (c); Bax (d); Bcl-2 (e); and Bcl-xL (f). Panels below the graphs show representative images of studied protein immunoblots. Results are presented as mean ± SEM of actin/studied protein ratio and referred to control cultures (c). Values were obtained from 4 independent experiments. between groups. C, control; CTK, cytokines; GHR, ghrelin; CTK + GHR, cytokines + ghrelin.

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