Methylation of Promoter Regions of Genes of the Human Intrauterine Renin Angiotensin System and Their Expression

Table 1

The percentage of gene alleles that were hypermethylated, intermediately methylated, and hypomethylated for ACE, AGTR1, ATP6AP2, CTSD, and KLK1 in placenta from women in early gestation (n = 4) or at delivery in late gestation in the absence of labour (n = 4, C/S).

Gene	Sample	Early gestation			C/S
		% Hypermethyl.	% Inter. methyl.	% Hypomethyl.	% Hypermethyl.	% Inter. methyl.	% Hypomethyl.
ACE	1	1.0	0.0	99.0	0.3	0.0	99.7
	2	—	—	—	0.8	0.0	99.2
	3	1.8	0.0	98.2	0.5	0.0	99.5
	4	0.7	0.0	99.3	0.7	0.0	99.3
AGTR1	1	1.8	0.0	98.2	0.3	0.0	99.7
	2	—	—	—	0.8	0.0	99.2
	3	1.5	0.0	98.5	0.2	0.0	99.8
	4	0.7	99.3	0.0	0.8	0.0	99.2
ATP6AP2	1	2.4	0.0	97.6	4.6	0.0	95.4
	2	7.4	92.4	0.2	1.0	0.0	99.0
	3	0.3	78.0	21.7	4.0	0.0	96.0
	4	—	—	—	0.7	0.0	99.3
CTSD	1	0.7	0.0	99.3	0.1	0.0	99.9
	2	—	—	—	0.3	0.0	99.7
	3	1.4	0.0	98.6	0.4	0.0	99.6
	4	0.1	99.8	0.1	0.2	0.0	99.8
KLK1	1	5.8	0.0	94.2	2.4	0.0	97.6
	2	6.4	93.6	0.0	5.8	0.0	94.2
	3	7.3	0.0	92.7	2.7	0.0	97.3
	4	0.0	100.0	0.0	2.5	0.0	97.5

Inter. = intermediate, methyl. = methylation. Samples with no value (—) had a DNA template in which ≥25% was digestion-resistant from combined methylation sensitive and resistant enzymes.