Insulin Increases Sestrin 2 Content by Reducing Its Degradation through the PI₃K/mTOR Signaling Pathway

Figure 1

Insulin treatment upregulates SESN2 protein levels in hepatic cells. HepG2 cells were incubated with indicated concentrations of insulin for 18 h (a and c) or 100 nM insulin for indicated time course (b). Cells were harvested for western blotting. In (a) and (b), HepG2 cells were cultured in DMEM containing 25 mM glucose. In (c), cells were grown with the indicated concentrations of glucose. (d) Primary mouse hepatic cells were treated with the indicated concentrations of insulin for 18 h, followed by cell harvesting and western blotting. Upper panels: western blot of SESN2 and β-actin (loading control); lower panel: densitometric analysis of two independent experiments. ; compared with noninsulin treatment.

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