Insulin Increases Sestrin 2 Content by Reducing Its Degradation through the PI₃K/mTOR Signaling Pathway

Figure 2

PI₃K/mTOR signaling pathway is required for the upregulation of SESN2 level by insulin treatment. HepG2 cells were pretreated with indicated reagents at the indicated concentrations for 1 h, followed by an 18 h further treatment with 100 nM of insulin. Cells were harvested for western blotting. (a) to (c) are representative images of at least three independent experiments.

(a)

(b)

(c)